Antigenic structure of outer membrane protein E of Moraxella catarrhalis and construction and characterization of mutants.

The Moraxella catarrhalis porin-like outer membrane protein CD is an adhesin for human lung cells.

The outer membrane protein CD (OMPCD) of Moraxella catarrhalis is an outer membrane protein with several attributes of a potential vaccine antigen. We isolated four transposon mutants of strain O35E on the basis of their reduced binding to A549 human lung cells in microcolony formation assays, and we determined that they contain a transposon in ompCD. We also found that these transposon inserti...

Molecular cloning and characterization of outer membrane protein E of Moraxella (Branhamella) catarrhalis.

Outer membrane protein E (OMP E) is a 50-kDa protein of Moraxella (Branhamella) catarrhalis. It is a potential vaccine antigen because it is expressed on the surface of the bacterium and has antigenic determinants which are conserved among most strains of M. catarrhalis. To clone the gene encoding OMP E, an EMBL-3 genomic library of strain 25240 was screened with a family of degenerate oligonuc...

Conservation of outer membrane protein E among strains of Moraxella catarrhalis.

Outer membrane protein E (OMP E) is a 50-kDa protein of Moraxella catarrhalis which has several features that suggest that the protein may be an effective vaccine antigen. To assess the conservation of OMP E among strains of M. catarrhalis, 22 isolates were studied with eight monoclonal antibodies which recognize epitopes on different regions of the protein. Eighteen of 22 strains were reactive...

Analysis of antigenic structure and human immune response to outer membrane protein CD of Moraxella catarrhalis.

Moraxella catarrhalis is an important cause of otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease (COPD). Outer membrane protein CD (OMP CD) is a 45-kDa protein which is a potential vaccine antigen to prevent infections caused by M. catarrhalis. Eight monoclonal antibodies were used to study the antigenic structure of the OMP CD ...

The outer membrane protein OlpA contributes to Moraxella catarrhalis serum